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Y chromosome diversity, human expansion, drift, and cultural evolution. Norfolk Island prohibits the importation of fresh fruit and vegetables, so much of the produce is grown locally by Biggs. They stress the laws are not the same as those introduced in the ACT and overturned by the High Court last year. Fragment lengths were accurately determined after detection by automated gel capillary electrophoresis. To facilitate more accurate and high throughput sequencing of complete mitochondrial genomes, we developed an in house method using a combination of long-range PCR and the Life Technologies Ion Torrent Next Generation Sequencing platform. Interestingly, we note that clade B4a1a1m in phylotree is defined by , and [ 16 ]; however, the clade we report B4a1a1a14 shares these variants as well as , potentially suggesting dubious positioning of the clade defined by Environment - current issues: While some Norfolk Islanders leave the island to go to university, find work or travel, and come back with an understanding about the Australian taxation and benefits system, many do not. Judging how many people are for or against the coming change is tricky, though. Negative controls were included in each PCR run to check for contamination and ensure adequate quality control.